Coding

Part:BBa_K2232000:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-17)


TSLV1-CA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 540
    Illegal NotI site found at 827
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 65
    Illegal SapI.rc site found at 223
    Illegal SapI.rc site found at 535


Design Notes

We use it as a coding protein for carbonic anhydrase


Source

GenBank: KR347171.1

References

Faridi S, Satyanarayana T. Characteristics of recombinant α-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1[J]. International Journal of Biological Macromolecules, 2016, 89:659-668.